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pseudotyped rabies virus  (Addgene inc)


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    Addgene inc pseudotyped rabies virus
    Ntsr1+ SC neurons input and output characterization. A , Photomicrographs of Cre-dependent <t>pseudotyped</t> rabies tracing of Ntsr1+ neurons. Starter cells in the SC are highlighted in magenta. Cell bodies were identified in the visual cortex, retina, pretectum, parabigeminal nucleus (PBG), and ventral lateral geniculate nucleus (vLGN). Three mice. B , Photomicrograph of the injection site and projection pattern of Ntsr1+ neurons in the midbrain after injection of an AAV carrying Cre-dependent tdTomato (Ntsr1+ neurons) together with an AAV carrying CAG-driven GFP (all SC neurons). The top row shows the injection site, and the bottom row shows projections in the thalamus. Scale bar, 100 µm, three mice. C , Photomicrograph of the injection site and projection pattern of Ntsr1+ neurons in the midbrain after injection of an AAV carrying Cre-dependent tdTomato only. D , Immunolabeling of the projections of neurons labeled in C for VGluT2 and synaptophysin. E , F , Same as C and D for the thalamus, showing the pulvinar and corresponding inputs from the Ntsr1+ SC neurons. Scale bars: C and D , 500 µm; D and F , 10 µm. G , Absolute quantification of putative synapses originating from Ntsr1+ neurons in the SC and their immunolabeling in the pulvinar and PBG. Wilcoxon signed rank test, p < 0.05, indicated with a star. H , Proportion of putative synapses labeled in the PBG and pulvinar. Four mice.
    Pseudotyped Rabies Virus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pseudotyped rabies virus/product/Addgene inc
    Average 93 stars, based on 29 article reviews
    pseudotyped rabies virus - by Bioz Stars, 2026-04
    93/100 stars

    Images

    1) Product Images from "Behavioral Modulation and Molecular Definition of Wide-Field Vertical Cells in the Mouse Superior Colliculus"

    Article Title: Behavioral Modulation and Molecular Definition of Wide-Field Vertical Cells in the Mouse Superior Colliculus

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.1816-24.2025

    Ntsr1+ SC neurons input and output characterization. A , Photomicrographs of Cre-dependent pseudotyped rabies tracing of Ntsr1+ neurons. Starter cells in the SC are highlighted in magenta. Cell bodies were identified in the visual cortex, retina, pretectum, parabigeminal nucleus (PBG), and ventral lateral geniculate nucleus (vLGN). Three mice. B , Photomicrograph of the injection site and projection pattern of Ntsr1+ neurons in the midbrain after injection of an AAV carrying Cre-dependent tdTomato (Ntsr1+ neurons) together with an AAV carrying CAG-driven GFP (all SC neurons). The top row shows the injection site, and the bottom row shows projections in the thalamus. Scale bar, 100 µm, three mice. C , Photomicrograph of the injection site and projection pattern of Ntsr1+ neurons in the midbrain after injection of an AAV carrying Cre-dependent tdTomato only. D , Immunolabeling of the projections of neurons labeled in C for VGluT2 and synaptophysin. E , F , Same as C and D for the thalamus, showing the pulvinar and corresponding inputs from the Ntsr1+ SC neurons. Scale bars: C and D , 500 µm; D and F , 10 µm. G , Absolute quantification of putative synapses originating from Ntsr1+ neurons in the SC and their immunolabeling in the pulvinar and PBG. Wilcoxon signed rank test, p < 0.05, indicated with a star. H , Proportion of putative synapses labeled in the PBG and pulvinar. Four mice.
    Figure Legend Snippet: Ntsr1+ SC neurons input and output characterization. A , Photomicrographs of Cre-dependent pseudotyped rabies tracing of Ntsr1+ neurons. Starter cells in the SC are highlighted in magenta. Cell bodies were identified in the visual cortex, retina, pretectum, parabigeminal nucleus (PBG), and ventral lateral geniculate nucleus (vLGN). Three mice. B , Photomicrograph of the injection site and projection pattern of Ntsr1+ neurons in the midbrain after injection of an AAV carrying Cre-dependent tdTomato (Ntsr1+ neurons) together with an AAV carrying CAG-driven GFP (all SC neurons). The top row shows the injection site, and the bottom row shows projections in the thalamus. Scale bar, 100 µm, three mice. C , Photomicrograph of the injection site and projection pattern of Ntsr1+ neurons in the midbrain after injection of an AAV carrying Cre-dependent tdTomato only. D , Immunolabeling of the projections of neurons labeled in C for VGluT2 and synaptophysin. E , F , Same as C and D for the thalamus, showing the pulvinar and corresponding inputs from the Ntsr1+ SC neurons. Scale bars: C and D , 500 µm; D and F , 10 µm. G , Absolute quantification of putative synapses originating from Ntsr1+ neurons in the SC and their immunolabeling in the pulvinar and PBG. Wilcoxon signed rank test, p < 0.05, indicated with a star. H , Proportion of putative synapses labeled in the PBG and pulvinar. Four mice.

    Techniques Used: Injection, Immunolabeling, Labeling, Quantitative Proteomics



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    Image Search Results


    Ntsr1+ SC neurons input and output characterization. A , Photomicrographs of Cre-dependent pseudotyped rabies tracing of Ntsr1+ neurons. Starter cells in the SC are highlighted in magenta. Cell bodies were identified in the visual cortex, retina, pretectum, parabigeminal nucleus (PBG), and ventral lateral geniculate nucleus (vLGN). Three mice. B , Photomicrograph of the injection site and projection pattern of Ntsr1+ neurons in the midbrain after injection of an AAV carrying Cre-dependent tdTomato (Ntsr1+ neurons) together with an AAV carrying CAG-driven GFP (all SC neurons). The top row shows the injection site, and the bottom row shows projections in the thalamus. Scale bar, 100 µm, three mice. C , Photomicrograph of the injection site and projection pattern of Ntsr1+ neurons in the midbrain after injection of an AAV carrying Cre-dependent tdTomato only. D , Immunolabeling of the projections of neurons labeled in C for VGluT2 and synaptophysin. E , F , Same as C and D for the thalamus, showing the pulvinar and corresponding inputs from the Ntsr1+ SC neurons. Scale bars: C and D , 500 µm; D and F , 10 µm. G , Absolute quantification of putative synapses originating from Ntsr1+ neurons in the SC and their immunolabeling in the pulvinar and PBG. Wilcoxon signed rank test, p < 0.05, indicated with a star. H , Proportion of putative synapses labeled in the PBG and pulvinar. Four mice.

    Journal: The Journal of Neuroscience

    Article Title: Behavioral Modulation and Molecular Definition of Wide-Field Vertical Cells in the Mouse Superior Colliculus

    doi: 10.1523/JNEUROSCI.1816-24.2025

    Figure Lengend Snippet: Ntsr1+ SC neurons input and output characterization. A , Photomicrographs of Cre-dependent pseudotyped rabies tracing of Ntsr1+ neurons. Starter cells in the SC are highlighted in magenta. Cell bodies were identified in the visual cortex, retina, pretectum, parabigeminal nucleus (PBG), and ventral lateral geniculate nucleus (vLGN). Three mice. B , Photomicrograph of the injection site and projection pattern of Ntsr1+ neurons in the midbrain after injection of an AAV carrying Cre-dependent tdTomato (Ntsr1+ neurons) together with an AAV carrying CAG-driven GFP (all SC neurons). The top row shows the injection site, and the bottom row shows projections in the thalamus. Scale bar, 100 µm, three mice. C , Photomicrograph of the injection site and projection pattern of Ntsr1+ neurons in the midbrain after injection of an AAV carrying Cre-dependent tdTomato only. D , Immunolabeling of the projections of neurons labeled in C for VGluT2 and synaptophysin. E , F , Same as C and D for the thalamus, showing the pulvinar and corresponding inputs from the Ntsr1+ SC neurons. Scale bars: C and D , 500 µm; D and F , 10 µm. G , Absolute quantification of putative synapses originating from Ntsr1+ neurons in the SC and their immunolabeling in the pulvinar and PBG. Wilcoxon signed rank test, p < 0.05, indicated with a star. H , Proportion of putative synapses labeled in the PBG and pulvinar. Four mice.

    Article Snippet: The viral titer was >10 12 GC/ml for AAV1.Flx.Split.TVA.B19G (catalog #52473, Addgene, RRID:Addgene_52473-AAV1) and >10 8 GC/ml for pseudotyped rabies virus (EnvA-coated G-deleted rabies-mCherry, Salk Institute, catalog #32636, Addgene, RRID:Addgene_32636).

    Techniques: Injection, Immunolabeling, Labeling, Quantitative Proteomics

    Key resources table

    Journal: The Journal of Neuroscience

    Article Title: Spinal Glycine Receptor Alpha 3 Cells Communicate Sensations of Chemical Itch in Hairy Skin

    doi: 10.1523/JNEUROSCI.1585-23.2024

    Figure Lengend Snippet: Key resources table

    Article Snippet: BRVenvA-1o Rabies Virus, pseudotyped EnvA, mCherry , pSADB19dG-mCherry , Charité, the material was originally provided by Edward Callaway and distributed through Addgene (plasmid #32630, #32631, #32632, #32633, #32634; ) , Charité , Unknown, with titer: 1.00 × 10 8 particles/ml , One site.

    Techniques: Plasmid Preparation, Injection, Virus, RNAscope